Fig. 3: Immune landscape and cellular distribution characteristics of postimmunotherapy oesophageal squamous cell carcinoma (ESCC).

A,B Representative cell segmentation maps were generated using the DeepCell algorithm for both the pathological complete response (PCR) and non-PCR groups. C Comparative analysis of absolute cell counts per unit area between the non-PCR (n = 10) and PCR (n = 10) groups. A t test was used to and p = 0.032 (two sided). The whiskers indicate the range from minimum to maximum values, and the bounds of the boxes represent the 25 and 75th percentiles. The centres denote the medians. D,E Two of ten patients for tissue architecture and spatial distribution of major immune cell subsets in both the non-PCR and PCR groups. F,G One of ten patients for expression patterns of functional markers in both the non-PCR and PCR groups.