Fig. 4: Optimization for fed-batch fermentation using 2AP strain.
From: High yield production of 3-hydroxypropionic acid using Issatchenkia orientalis
The profile of the 2AP strain for glucose consumption (A), pyruvate accumulation (B), and 3HP production (C) in both the CSM medium and the CSL medium in shake flask. Data present mean ± S.D. of three biologically independent samples (n = 3). D fed-batch fermentation a 300 mL. Glucose feeding began at 24 h post inoculation. The 2AP strain was used for fermentation. During fermentation, pyruvate, and glucose were monitored. No pyruvate accumulation or other metabolites greater than 1 g/L were observed. The cultivation was set at DO ranging from 5% to 15% and pH at 4 with CO2 gassing. For fed-batch fermentation, two independent biological replicates were performed (n = 2) and mean value from two replicates is presented. Source data for this Figure is available in the Source Data file. Created in BioRender. Zhao, H. (2025) https://BioRender.com/vkz7w01.
