Table 1 Thermodynamic analysis of the β-alanine pathway

From: High yield production of 3-hydroxypropionic acid using Issatchenkia orientalis

Gene

Reaction Name

ΔrG’°, pH7

ΔrG’°, pH6

  

dGpredictor

PYC

pyruvate carboxylase

−4.99 ± 0.10

−1.72 ± 0.10

AAT

aspartate transaminase

3.01 ± 0.10

3.16 ± 0.10

PAND

aspartate 1-decarboxylasea,b

−22.49 ± 0.23

−28.06 ± 0.23

BAPAT

β-alanine-pyruvate aminotransferasea,c

−3.28 ± 0.14

−3.27 ± 0.14

YDFG

malonic semialdehyde reductasea,d

−23.89 ± 0.14

−29.66 ± 0.14

  1. Data presents the estimated changes in Gibbs free energy, ΔrG’° (kJ/mole) ± standard error (kJ/mole) with both values calculated using the dGpredictor computational tool.
  2. anon-native.
  3. bcatalyzes decarboxylation of aspartate to β-alanine.
  4. cconverts β-alanine into malonic semialdehyde (MSA).
  5. dconverts MSA to 3HP.
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