Fig. 2: Generations of SunTag Plug-in ABE8eWQ and SunTag Plug-in miniCGBE1.
From: A Plug-in system for reprogramming the editing patterns of base editors
A Schematic of ABE8eWQ and SunTag Plug-in ABE8eWQ (D10A-GCN4 and AntiGCN4-ABE8eWQ). B Editing window analysis of SunTag Plug-in ABE8eWQ. Window graph is obtained by merging the edit results at HEK site 2, E21, ZAP70, and OCT4. Numbers with different color represent D10A-GCN4 variants. Editing window of ABE8eWQ serves as control group (n = 2 biological replicates). Data are mean ± SD (Blue group, n = 5 variants; Green group, n = 14 variants; Purple group, n = 10 variants; Yellow group, n = 23 variants; Gray group, n = 10 variants). C Assessment of single-base editing precision for selected variants (n = 2 biological replicates). The legend only A5-to-G indicates that only the fifth A on 20nt spacer is edited to G, and the spacer does not contain any other mutation. Other legend naming rules are analogies. D Schematic of miniCGBE1 and SunTag Plug-in miniCGBE1 (D10A-GCN4 and AntiGCN4-miniCGBE1). E Editing window analysis of SunTag Plug-in miniCGBE1. The Window graph was obtained by merging the editing results of FANCF site 4, EMX1, PPP1R12C, and HEK site 3. Certain cytosines at X-axis coordinates are specifically labeled with motif. Numbers with different color represent D10A-GCN4 variants. Editing window of miniCGBE1 serves as control group (n = 2 biological replicates). Data are mean ± SD (Blue group, n = 12 variants; Green group, n = 13 variants; Purple group, n = 3 variants; Yellow group, n = 25 variants; Gray group, n = 9 variants). F, G Editing product analysis of high-efficiency D10A-GCN4 variants of SunTag Plug-in miniCGBE1 at FANCF site 4 and PPP1R12C, and its editing precision analysis (n = 2 biological replicates). Source data are provided as a Source Data file.
