Fig. 5: Effect of reordering antibody and deaminase on editing outcome in Plug-in ABE8eWQ and Plug-in ABE9.
From: A Plug-in system for reprogramming the editing patterns of base editors
A Schematic of different orderings in Plug-in ABE8eWQ. B Impact of different orderings in Plug-in ABE8eWQ framework on editing windows (n = 2 biological replicates). Window is obtained by combining the editing results at HEK site 2, E21, ZAP70, and OCT4. 532GCN4-ABE8eWQ represents the combination of D10A-532GCN4 and AntiGCN4-ABE8eWQ. Other combinations are named in analogies. C, D Product analysis and precision analysis for different orderings in Plug-in ABE8eWQ framework at four gene loci (n = 2 biological replicates). E Schematic of different orderings in Plug-in ABE9. F Impact of different orderings in Plug-in ABE9 framework on editing windows (n = 2 biological replicates). G, H Product analysis and precision analysis for different orderings in Plug-in ABE9 framework at four gene loci (n = 2 biological replicates). Source data are provided as a Source Data file.
