Fig. 1: TMEM65 is a conserved protein of the IMM.
From: TMEM65-dependent Ca2+ extrusion safeguards mitochondrial homeostasis
a Cladogram showing the phylogenetic distribution of MCU (red) and TMEM65 (green) homologs across 1088 reference proteomes. b Schematic representation of TMEM65 membrane topology. Topological structure was generated using Protter40. c Western blot showing expression of TMEM65 and TME65-3xFlag in HeLa cells. Comparable results were obtained in at least three independent transfections. d Immunolocalization of TMEM65-3xFlag (cyan) and the mitochondrial marker SDHA (magenta) in HeLa cells, scale bar is 10 μm. Comparable staining was obtained in at least three independent transfections and with other mitochondrial markers. e Proteinase K protection assay performed in mitoplasts isolated from TMEM65-3xFlag-expressing HeLa cells. Comparable results were obtained in at least three independent experiments.
