Fig. 3: GlpG catalysis is required for bacterial invasion by commensal and uropathogenic strains in bladder and kidney cell lines. | Nature Communications

Fig. 3: GlpG catalysis is required for bacterial invasion by commensal and uropathogenic strains in bladder and kidney cell lines.

From: Rhomboid protease GlpG regulates type 1 pili quality control and virulence in pathogenic E. coli

Fig. 3: GlpG catalysis is required for bacterial invasion by commensal and uropathogenic strains in bladder and kidney cell lines.

a Gentamicin protection assay used to quantify levels of invasion between different strains of E. coli. Created with BioRender.com. https://BioRender.com/bl2bx7c. b Quantification of infection between different strains of E. coli against mIMCD-3, mouse kidney cell line, and c T24, human bladder epithelial cell line reported as mean Colony Forming Units (CFU) per mL. Epithelial cells were infected using wild-type commensal K12 substr. MG1655 (green), or UPEC NU149 strains (blue) and their glpG mutants. Each strain was performed in biological and technical triplicates and with an n = 9. Data are presented as mean values +/− SEM. Significant differences between data sets were determined using one-way ANOVA.

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