Fig. 7: mGluR4 and Npdc1 form a physical complex and act synergistically to modulate signaling.
From: mGluR4–NPDC1 complex mediates α-synuclein fibril-induced neurodegeneration
a Representative immunoblots with anti-V5 and anti-DYDDK of co-IP assays using HEK293T cells expressing GRM4-DYDDK or GRM5-DYDDK, together with V5-tagged NPDC1. b Graphs show mean ± SEM of pulldown by V5 beads (left) and DYDDK beads (right). N = 3 independent experiments. One-way ANOVA with Tukey’s post hoc test. For both V5 and DYDDK pulldown: ***p < 0.0001 for GRM4-DYDDK vs NPDC1-V5 + GRM4-DYDDK and NPDC1-V5 vs GRM4-DYDDK + NPDC1-V5. c Graph shows mean ± SEM of calcium influx curve after L-AP4 stimulation in GRM4-Gαqi5 cells (G4) transfected with an empty vector (Vec, pcDNA) or with NPDC1. N = 3 mean values from 3 independent experiments, each mean value calculated from 3 wells. d (left) Graph shows mean ± SEM of calcium influx curve after L-AP4 stimulation in GRM4-Gαqi5 cells (G4) co-cultured with HEK293T cells transfected with an empty vector (Vec, pcDNA) or with NPDC1 at different ratios. d (right) Graph shows mean ± SEM of calcium response after 100 µM L-AP4 stimulation in GRM4-Gαqi5 cells (G4) co-cultured with HEK293T cells transfected with an empty vector (Vec, pcDNA) or with NPDC1 at different ratios. N = wells numbers from 3 independent experiments shows above the bar. One-way ANOVA with Tukey’s post hoc test. ***p = 0.0009 for G4:Vec, 1:1 vs G4:NPDC1, 1:1; **p = 0.0015 for G4:Vec, 1:2 vs G4:NPDC1, 1:2.
