Fig. 8: mGluR4 and Npdc1 synergistically drive synaptic loss in cortical neurons.
From: mGluR4–NPDC1 complex mediates α-synuclein fibril-induced neurodegeneration
a Representative images of streptavidin (PFF, green), MAP2 (red), and DAPI (blue) in cultured neurons from WT, dHET and dKO mice after 2 h of exposure with biotinylated α-syn monomer or PFF at 37 °C. Scale bar, 50 µm. b Representative images of p-α-syn (green), MAP2 (red) and DAPI (blue) in cultured neurons from WT, dHET and dKO mice after 7 days of exposure with α-syn monomer or PFF at 37 °C. Scale bar, 50 µm. c Representative images of Synapsin1 (green), Homer1/2 (red), and DAPI (blue) staining in cultured neurons from WT, dHET and dKO mice after 7 days of exposure with α-syn monomer or PFF at 37 °C. Scale bar, 50 µm. d Quantification of streptavidin (PFF) fluorescence intensity within MAP2-positive regions after 2 h of exposure with biotinylated a-syn monomer or PFF at 37 °C. Data represent mean ± SEM from WT(N = 5), dHET(N = 6), and dKO(N = 5) embryos. One-way ANOVA with Tukey’s post hoc test. 150 nM: *p = 0.018 for WT vs dHET, *p = 0.012 for WT vs dKO; 300 nM: ***p < 0.0001 for WT vs dHET and WT vs dKO; 600 nM: ***p < 0.0001 for WT vs dHET and WT vs dKO. e Quantification of p-α-syn fluorescence intensity within MAP2-positive regions after 7 days of exposure with a-syn monomer or PFF. Data represent mean ± SEM from WT(N = 5), dHET(N = 6), and dKO(N = 5) embryos. One-way ANOVA with Tukey’s post hoc test. 150 nM: *p = 0.034 for WT vs dHET, *p = 0.028 for WT vs dKO; 300 nM: ***p < 0.0001 for WT vs dHET and WT vs dKO; 600 nM: ***p < 0.0001 for WT vs dHET and WT vs dKO. f Quantification of Synapsin1 and Homer1/2 double positive dots within MAP2-positive regions after 7 days of exposure with a-syn monomer or PFF. Data represent mean ± SEM from WT(N = 5), dHET(N = 6), and dKO(N = 5) embryos. One-way ANOVA with Tukey’s post hoc test. 300 nM: *p = 0.040 for WT vs dHET; 600 nM: *p = 0.039 for WT vs dHET, **p = 0.0014 for WT vs dKO.
