Fig. 1: Measuring the dynamics of betaxanthin-producing single cells. | Nature Communications

Fig. 1: Measuring the dynamics of betaxanthin-producing single cells.

From: Exploring single-cell biosynthetic noise and dynamics for enhanced betaxanthin production in Escherichia coli

Fig. 1: Measuring the dynamics of betaxanthin-producing single cells.

a The betaxanthin biosynthetic pathway. L-DOPA is provided to the cell media and is converted to betalamic acid by DOD which is fused to mCherry. Betalamic acid spontaneously reacts with primary amines to form yellow fluorescent betaxanthins. b Microfluidics chip and representative image of a single chamber within the microfluidics chip where the cells were maintained at the steady state. P1 is the inlet of cell culture, P2 is the inlet of fresh media, and P3 is the outlet of waste. c Representative time-lapse fluorescence microscopy images for measuring single-cell growth (top), protein level (middle), and metabolite level (bottom). Source data are provided as a Source Data file.

Back to article page