Fig. 2: Biogenesis of initiation and termination factors.
From: Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery
a Top: IF assembly on the ribosomal subunit during early stages of translation. Bottom: Translation termination with release and recycling factors. b Top: FI(t) from ΔIF-PURE reactions (ΔIFs and ΔMTF), with or without the corresponding IF genes. For each IF: [IF DNA] = 0.1 nM; [GFP DNA] = 0.4 nM. Bottom: Biogenesis assay in ΔRF-PURE, (ΔRF1, ΔRF3, and ΔRRF) ± RF genes. For each RF: [RF DNA] = 0.1 nM; [GFP DNA] = 0.4 nM. All FI values are normalized to the maximum FI of a full PURE reaction. Mean ± STD (n = 3, independent experiment). c Bottom: Difference between the normalized max. rate ± their corresponding genes (Δkmax) of ΔPURE for all the permutations of the three IFs and MTF (minimum n = 3 independent experiments). Dotted horizontal lines: guide to the eye for comparing the recovery levels of each subgroups. Top: the time point of the max. FI″(t), tmax, indicating the moment the threshold concentration is crossed due to biogenesis. d Bottom: Δkmax for ΔPURE reaction with all the permutations of a grouping of RF1, RF3, and RRF± their corresponding gene with n = 3 independent experiments. Top: corresponding times max. FI”(t) Source data are provided as a Source Data file.
