Fig. 6: Biogenesis of all translation proteins from DNA brushes.
From: Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery
a Scheme: biogenesis for all translation factors from a minimal translation system (see Supplementary Table 2). b Gene ratios within the DNA brush (see Supplementary Table 3). c TIRF microscopy images of the synthesis of all translation proteins from surface-immobilized DNA brushes. Top row: DNA brush with a gene ratio of 2% coding for GFP-HA and 98% for a non-translation (“dummy”) protein (see b)(DNA not labeled). Bottom row: DNA brushes with a gene ratio of 90% coding for all translation genes (DNA-labeled), 2% coding for GFP-HA and 8% for dummy protein (see b). Upon exposure to a protein synthesis reaction at threshold, surface-bound GFP-HA accumulates around DNA brushes only with translation genes. Scale bar = 200 µm. d Average GFP FI(t) for DNA brushes with different fractions of translation genes. Repeats in Supplementary Fig. 28. e Mean fold change of GFP FI ± s.e.m from DNA brushes with different amount of translation genes (normalized to the signal from “0%” brush) at = 6 h and its s.e.m. as a function of the DNA FI (translation protein-DNA + GFP DNA) for different DNA brushes (n = 6 DNA brush cluster from two different slides). Source data are provided as a Source Data file.
