Fig. 5: Promoting effect of synthetic microbiota on ECMF growth.
From: Mycorrhizal colonization of dryland tree establishment depends on soil microbial cooperation
a the effects of synthetic microbiota on Tuber growth were tested by three methods. SynM, a synthetic microbial community comprising of the nine key bacterial strains. SynM1, three strains from the SynM with neutral effects on Tuber growth. SynM2, six strains from the SynM exhibiting Tuber growth promotion. SynM3, nine randomly selected strains corresponding to ectomycorrhizosphere-enriched ASVs. Bar charts represent mean ± SD; Different lowercase letters indicate significant differences (one-way ANOVA followed by Tukey test, P < 0.05; n = 4 biological replicates, in triplicates); b comparison of three methods for Tuber growth promotion by the rationally designed 11-strain SynM inoculation. Bar charts represent mean ± SD; Different lowercase letters indicate significant differences (one-way ANOVA followed by Tukey test, P < 0.05; n = 4 biological replicates, in triplicate). c a bacterial strain attached to Tuber hyphae. d KEGG pathway analysis for upregulated genes, ranked by rich factor (FDR < 0.05 cut-off). e Heat map and butanoate metabolism pathway for upregulated genes (KEGG). SynM (1–3) denotes three biological replicates of the SynM treatment group.
