Fig. 3: Survival and epigenetic age acceleration (EAA) in singletons.

One women was randomly selected from each twin pair, and the estimates (shown as individual points) were collected across 100 different models with random singleton subsets. The black lines with error bars represent the aggregated estimates with 95% confidence intervals (CI) across all 100 models calculated by a fixed-effects meta-analysis. a-c. Mortality hazard ratio estimates for each class from a Cox proportional hazards model weighted with class-specific posterior probabilities and adjusted for left-truncation, historical cohort (a n = 9496 women per model), and further adjusted for risk factors (alcohol use, smoking, body mass index, education) with imputed data (b n = 9496 per model), and non-imputed data (c n_Mean=6031.15, n_Range = 6011–6054 per model, sample sizes vary due to random selection of individuals with or without risk factor data in each of the 100 draws). PCGrimAge EAA estimates for each class from a Bolck-Croon-Hagenaars model adjusted for age (d n = 611 women), with additional adjustments for risk factors (e n = 611 women). The dashed red line is drawn for comparison to survival analysis at the estimates for class 3. Source data are provided as a Source Data file.