Fig. 4: Enzymatic conversion through stepwise and one-pot cascade reactions.
From: Systemic engineering and global regulation enabling high-level bilirubin biosynthesis
a Time-course analysis of biliverdin (BV) production from heme using wild-type enzymes and optimized truncation variants (RnHO-1Δ22/RnCPRΔ54), demonstrating enhanced catalytic performance of the engineered pair, data are presented as means ± SD with error bars connected sequentially to form error bands. (n = 3 biological replicates). b Time-dependent conversion of BV to bilirubin (BR) catalyzed by RnBVR, confirming efficient reductase activity, data are presented as means ± SD with error bars connected sequentially to form error bands. (n = 3 biological replicates). c One-pot enzymatic cascade converting heme to BR using the optimized RnHO-1Δ22/RnCPRΔ54 pair and RnBVR, illustrating seamless integration of the two-step pathway for bilirubin biosynthesis, data are presented as means ± SD with error bars connected sequentially to form error bands. (n = 3 biological replicates). Source data are provided as a Source Data file.
