Fig. 5: Functional identification of the TS genes in P. chienii. | Nature Communications

Fig. 5: Functional identification of the TS genes in P. chienii.

From: Analysis genome of Pseudotaxus chienii reveals insights into the origin and evolution of taxane biosynthesis

Fig. 5

a Phylogenetic analysis of the TPS family members in different plants. Purple stars indicated the TPS from P. chienii (PcTS1/2). Green dots indicated the TPS containing Class I (DDXXD/E) motif, red dots indicated the TPS containing Class II (DXDD) motif, and yellow dots indicated the TPS containing the bifunctional motif (DDXXD/E + DXDD). b Identification of positive transgenic tobacco expressing PcTS1 and TwTS1, respectively. c GC–MS analysis of hexane extracts from the seedlings expressing PcTS1 and TwTS1, and controls (CK), respectively. Time (11.857) indicates the target peak of taxadiene. d Mass spectra profile of the compound at 11.857 min matched exactly with the taxadiene mass spectra. e Multiple sequence alignment analysis of the DXDD and DDXXD/E motifs from different TPS proteins. f Structure modeling of PcTS1 by the SWISS-MODEL program using 3p5p.1.A model as template. The bioactive center of PcTS1 zoom in through a red box. g Protein structure analysis of the typical TPSs from the Angiosperm (Class I), Angiosperm (Class II), and other gymnosperms (bifunctional TPS), respectively. Source data are provided as a Source Data file.

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