Fig. 1: PTRH2 mRNA and protein is downregulated in PPCM female patient hearts. | Nature Communications

Fig. 1: PTRH2 mRNA and protein is downregulated in PPCM female patient hearts.

From: Peptidyl-tRNA hydrolase 2 is a negative regulator of peripartum cardiomyopathy with heart failure in female mice

Fig. 1

a–c PTRH2, ACTA1, and MYH7 mRNA quantitation from female normal donor and PPCM LV samples. Data shown is from biologically independent human samples/group. PTRH2 (n = 4; p = 0.001), ACTA1 (n = 5; p = 0.00073), and MYH7 (n = 5; p = 0.0198). d, e Immunoblot (left) and protein quantification (right) of PTRH2 (n = 5; p < 0.0001) and cardiomyopathy markers ACTA1(n = 5; p < 0.0001) and β-MHC (n = 5; p = 0.003) in normal donor and PPCM LV samples. Data shown is from biologically independent human LV samples/group. Statistical test (a–e) was by Two-tailed unpaired t-test. Error bars are mean + s.e.m. Each dot on graph represents an independent biological LV sample from either normal donor or PPCM. f Normal donor or PPCM LV sections immunostained for PTRH2. Scale bar, 50 μm. g Experimental workflow for RNA-seq on normal donor and PPCM LV heart samples. h Differential gene expression (DEG) from RNA-seq analysis of PPCM compared to normal donor LV heart samples. i Heat map of 50 DEGs (row normalized) in PPCM versus normal donor LV samples; increased (red), decreased (green), baseline value (black). j STRING network map of up- and down-regulated genes identified in PPCM/DCM, Cardiac Hypertrophy, PI3K/AKT and Integrin signaling pathways in PPCM LVs compared to normal donor LVs. Source data are provided as a Source Data file. Schematic in g was created using BioRender (https://biorender.com). See Supplementary Fig. 1.

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