Fig. 3: Cardiomyopathy markers are downregulated in Ptrh2-CKO Postpartum hearts. | Nature Communications

Fig. 3: Cardiomyopathy markers are downregulated in Ptrh2-CKO Postpartum hearts.

From: Peptidyl-tRNA hydrolase 2 is a negative regulator of peripartum cardiomyopathy with heart failure in female mice

Fig. 3

a–d mRNA expression of hypertrophic and cardiomyopathy marker genes (Nppa, Nppb, Acta1, and Myh7) in LV heart samples from Ptrh2-NT and Ptrh2-CKO NP and PP groups. a Nppa: n = 5 (p = 0.0339) biologically independent mice/genotype/group; b Nppb: n = 7 (p = 0.0037) biologically independent mice/genotype/group, (c) Acta 1: n = 5 (p = 0.0082) biologically independent mice/genotype/group and (d) Myh7: n = 7 (p = 0.0362) biologically independent mice/genotype/group. Statistical test (a–d) by Two-Way ANOVA with Tukey’s multiple comparison performed in triplicate. Error bars are mean± s.e.m. Immunoblot (left) and quantification (right) of Acta1(e; n = 3, p = 0.0163) and β-Mhc (f; encoded by the Myh7 gene; n = 5; p = 0.0158) in LV samples normalized to Gapdh from biologically independent mice/genotype/group. Statistical test (e, f) by Two-Way ANOVA with Tukey’s multiple comparison performed in triplicate. Error bars are mean± s.e.m. g Experimental workflow for RNA-seq of Postpartum LV heart samples (Ptrh2-NT versus Ptrh2-CKO). h DEGs from RNA-seq analysis of Postpartum LV heart samples (Ptrh2-NT versus Ptrh2-CKO). i KEGG pathway enrichment. j Heat map of 100 DEGs (row normalized); upregulation (orange); downregulation (blue); baseline value (white). k STRING network map of up- and down-regulated genes identified in PPCM/DCM, Cardiac Hypertrophy, Pi3k/Akt and integrin signaling pathways in Ptrh2-NT versus Ptrh2-CKO LVs. Source data provided as a Source Data file. Schematic in (g) was created using BioRender (https://biorender.com). See Supplementary Fig. 3.

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