Fig. 6: Oscillator-Based Computation: Phase Locking in Coupled CRISPRlators.
From: Intracellularly coupled oscillators for synthetic biology
a Schematic of intracellularly coupled oscillators, where small chemical inputs drive binary outputs based on phase differences. b Correlation matrix showing in-phase and anti-phase signals, with an example AND gate operation. c, e, g, i Circuit topologies (top) and protein oscillations (bottom), demonstrating phase locking with low dCas concentration (200 molecules). d, f, h, j Correlation matrices computed from protein time series. k Schematic representation of an alternative (explicit) coupling strategy between oscillators. l The effect of the repression strength between the oscillators (β) to the phase-locking. In panels (l) and (n), the simulations were run for 105 min, with the first 15% treated as transient. Parameters were a1,N2 = 1.1, a1,N6 = 1.2, and in (n) [dCas] = 200 molecules. m Schematic illustration of implicit coupling between oscillators mediated by the time-dependent burden. n Phase-locking value versus dCas concentration for various half-saturation constants K; lower K indicates stronger burden-mediated coupling. The details of the simulations are available in Supplementary Data 15. An extended figure caption with additional details is available in Supplementary Note 5.1. The figure can be reproduced using the computer_CRISPRlator.m, computer_CRISPRlator_sctoch_burden.m and computer_CRISPRlator_sctoch_repress.m scripts.
