Fig. 3: Gamma counting and autoradiography confirm increased 18F-FMD uptake in spinal cords from EAE mice. | Nature Communications

Fig. 3: Gamma counting and autoradiography confirm increased 18F-FMD uptake in spinal cords from EAE mice.

From: A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitis

Fig. 3: Gamma counting and autoradiography confirm increased 18F-FMD uptake in spinal cords from EAE mice.

a Ex vivo gamma counting results (%ID/g) in whole brains, cervical/thoracic spinal cords, and lumbar spinal cords of naïve (n = 9), pre-EAE (n = 5), and EAE mice (n = 6). b Representative high-resolution ex vivo digital autoradiography of 60 µm-thick fresh frozen spinal cord sections (representative images based on mean spinal cord uptake) with H&E staining overlay. c Representative images at 40x magnification of H&E and CD68 immunohistochemical (IHC) staining of 12 µm lumbar spinal cord sections from naïve, pre-EAE, and EAE mice. Scale bars, 50 µm. C/T cervical/thoracic, L lumbar, SC spinal cord. a Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test. +versus naïve mice and *denotes direct comparison of groups. a C./T. SC: p = 0.0001 for naïve vs. EAE, p = 0.0022 for pre-EAE vs. EAE n = 5/group were stained for H&E and CD68 IHC. Data are mean ± SD.

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