Fig. 6: Flow cytometry reveals increased numbers of immune cells in spinal cord and blood from EAE mice and that immune expansion and infiltration is significantly reduced following treatment with H74DS3M8 or FTY720. | Nature Communications

Fig. 6: Flow cytometry reveals increased numbers of immune cells in spinal cord and blood from EAE mice and that immune expansion and infiltration is significantly reduced following treatment with H74DS3M8 or FTY720.

From: A radiolabeled dendrimer non-invasively identifies and tracks innate immune cell activation in a mouse model of experimental autoimmune encephalomyelitis

Fig. 6: Flow cytometry reveals increased numbers of immune cells in spinal cord and blood from EAE mice and that immune expansion and infiltration is significantly reduced following treatment with H74DS3M8 or FTY720.

a EAE was induced in C57BL/6J mice, and a group of them was treated with either H74DS3M8, FTY720, or vehicle. Created in BioRender. Reyes, S. (2026) https://BioRender.com/57g010x. Blood was collected and spinal cords were harvested for single-cell flow cytometry at different disease states. Summarized proportion of infiltrating macrophages, resting microglia, monocytes/dendritic cells, neutrophils, T cells, and B cells in the b spinal cord and c blood of naïve, pre-EAE, EAE with vehicle, EAE with H74DS3M8, and EAE with FTY720 mice. b, c Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons tests. +versus naïve mice, #versus H74DS3M8-treated EAE mice, and *denotes direct comparison of groups. Resting microglia, activated microglia, DCs, neutrophils, monocytes, T and B cells (blood): n = 5/group; infiltrating macrophages: n = 11 naïve, n = 9 pre-EAE, n = 18 EAE+vehicle, n = 6 EAE + H74, n = 5 EAE + FTY720; T cells (spinal cord): n = 11 naïve, n = 9 pre-EAE, n = 16 EAE + vehicle, n = 9 EAE + H74, n = 5 EAE + FTY720; B cells (spinal cord): n = 8 naïve, n = 9 pre-EAE, n = 15 EAE + vehicle, n = 9 EAE + H74, n = 5 EAE + FTY720. b p < 0.0001 for all groups except infiltrating macrophages: p = 0.048 naïve vs. EAE + H74, neutrophils: p = 0.028 naïve vs. EAE + H74, T cells: p = 0.003 naïve vs. EAE + H74, p = 0.017 pre-EAE vs. EAE + H74, p = 0.03 EAE + H74 vs. EAE + FTY720; c p < 0.0001 for all groups except monocytes: p = 0.0007 naïve vs. pre-EAE, p = 0.03 pre-EAE vs. EAE + FTY720; neutrophils: p = 0.02 naïve vs. EAE + H74, T cells: p = 0.0001 naïve vs. pre-EAE, p = 0.0016 pre-EAE vs. EAE+vehicle, p = 0.0055 pre-EAE vs. EAE + H74, p = 0.048 EAE + H74 vs. EAE + FTY720; B cells: p = 0.0001 naïve vs. EAE + H74, p = 0.0004 pre-EAE vs. EAE+vehicle, p = 0.003 pre-EAE vs. EAE + FTY720, p = 0.0014 EAE + vehicle vs. EAE + H74; p = 0.0008 EAE + H74 vs. EAE + FTY720. H74 = H74DS3M8. Data are mean ± SD. Parts of (a) created in BioRender.

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