Fig. 7: Cy5-labeled hydroxyl dendrimers are specifically endocytosed by activated myeloid cells in EAE mice and extent of uptake reflects PET imaging findings using ¹⁸F-FMD.

Percentage of Cy5-HD⁺ cells in a spinal cord and b blood of naïve, pre-EAE, EAE with vehicle, EAE with H74, and EAE with FTY720 mice. a, b Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons tests. +versus naïve mice, #versus H74DS3M8-treated EAE mice, and *denotes direct comparison of groups. Activated microglia, DCs, neutrophils, monocytes: n = 5/group; infiltrating macrophages: n = 12 naïve, n = 8 pre-EAE, n = 15 EAE+vehicle, n = 8 EAE + H74, n = 5 EAE + FTY720. a p < 0.0001 for all groups; b p < 0.0001 for monocytes: naïve vs. pre-EAE, naïve vs. EAE + vehicle, pre-EAE vs. EAE + vehicle, EAE+vehicle vs. EAE + H74, EAE + vehicle vs. EAE + FTY720; monocytes: p = 0.0083 naïve vs. EAE + H74, p = 0.01 naïve vs. EAE + FTY720, p = 0.01 pre-EAE vs. EAE + H74, p = 0.006 pre-EAE vs. EAE + FTY720; p < 0.0001 for neutrophils: naïve vs. pre-EAE, naïve vs. EAE + vehicle, pre-EAE vs. EAE + H74, pre-EAE vs. EAE + FTY720, EAE + vehicle vs. EAE + H74, EAE+vehicle vs. EAE + FTY720; neutrophils: p = 0.003 naïve vs. EAE + H74. H74 = H74DS3M8. Data are mean ± SD.