Fig. 7: Ligand–receptor interaction analysis between the IL-36γ-expressing keratinocytes and neutrophils in GPP skin.

A Cell–cell interaction number analysis among IL36G- Spinous, IL36G+ Spinous, IL36G- Supraspinous, IL36G+ Supraspinous, and neutrophils in control and GPP group. B The incoming and outgoing signaling strength of different subtypes in control and GPP group. C The significant signaling pathways were ranked based on their differences in overall information flow within the inferred networks between control and GPP group. D Cellular interactions of VEGI signaling pathway in GPP group. E Signal pair interactions from neutrophils to keratinocyte subtypes identified with CellChat analysis in GPP group. F Feature plots showing expression of TNFSF15 in myeloid cells. G Spatial transcriptome image of lesional sections showing the co-localization of IL36G + TNFRSF25+ differentiated keratinocytes and TNFSF15 + MPO+ neutrophils. The pink frame represents differentiated keratinocyte. The green frame represents myeloid cell. The size bar represents 500 µm (left, zoom out) or 100 µm (right, zoom in). H Schematic of the experimental design for the in vitro coculture assay. Created in BioRender. Jiang, R. (2025) https://BioRender.com/lcyuhhl<https://urldefense.com/v3/__https://BioRender.com/lcyuhhl__;!!NnSTv5QBqPjS9UMk!JEO9XaAu3G1endKkUtPBlL_enDqU_76Al307YLCYnUt2lDpHO_kWRIx-Yn_M0xHteCCZv_1plokWETudQJQZeRZYkw$>. I qRT-PCR of inflammatory genes in primary keratinocytes after co-culture with neutrophils (n = 3 biologically independent samples; one-way ANOVA; mean ± SEM). J qRT-PCR of inflammatory genes in WT/IL36G KO N/TERTs after co-culture with neutrophils (n = 3 biologically independent samples; one-way ANOVA; mean ± SEM).