Fig. 4: Conserved inflammatory signature in BNCT-treated tumour cells revealed by single-cell RNA sequencing. | Nature Communications

Fig. 4: Conserved inflammatory signature in BNCT-treated tumour cells revealed by single-cell RNA sequencing.

From: Boron neutron capture therapy preserves immune cells and induces robust anti-tumour immunity in preclinical mouse model

Fig. 4: Conserved inflammatory signature in BNCT-treated tumour cells revealed by single-cell RNA sequencing.The alternative text for this image may have been generated using AI.

a Workflow for single-cell RNA sequencing of B16F10 tumours (C57BL/6J mice, 6–8-week-old, female). b UMAP plot of all single-cell transcriptomes from 6 B16F10 samples coloured by major cell types. c Gene Ontology (GO) analysis of upregulated genes in BNCT-treated B16F10 cancer cells compared with X-ray (4 Gy)-treated B16F10 cancer cells on days 1 and 7. Fisher’s exact test. d UMAP plot of all cancer cell transcriptomes from 6 B16F10 samples coloured by cell subtype. e Proportional changes in c3 cancer cell subtypes. NC, negative control. f Number of genes expressed across the four cancer cell subtypes in the B16F10 samples. Mann-Whitney-Wilcoxon test two-sided, ****p < 0.0001. Top and bottom quartiles are shown with horizontal lines at the median, and whiskers denote the range of the data that falls within 1.5 times the interquartile range above the third quartile and below the first quartile. c0, 6418 cells; c1, 4977 cells; c2, 4947 cells; c3, 3342 cells. g Top 10 enriched GO pathways in the c3 subtypes of BNCT-treated B16F10 tumour cells. Fisher’s exact test. h Workflow for single-cell RNA sequencing of MC38 tumours (C57BL/6J mice, 6–8-week-old, female). i UMAP plot of all single-cell transcriptomes from 6 MC38 samples coloured by major cell types. j Boxplot of the expressed genes number across four cancer cell subtypes. Fisher’s exact test. k Proportion of four cancer cell subtypes. l Expressed genes number across four cancer cell subtypes in MC38 samples. Mann-Whitney-Wilcoxon test two-sided, ****p < 0.0001. Top and bottom quartiles are shown with horizontal lines at the median, and whiskers denote the range of the data that falls within 1.5 times the interquartile range above the third quartile and below the first quartile. c0, 7986 cells; c1, 813 cells; c2, 5846 cells; c3, 6047 cells. m Top 10 enriched GO pathways in the c3 subtypes of BNCT-treated MC38 tumour cells. Fisher’s exact test. n Venn diagram showing the overlap of differentially expressed genes in c3 cancer cells from B16F10 and MC38 tumour tissues.

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