Fig. 2: Isolation of PYR1nita, a nanomolar sensor for the synthetic opioid nitazene.
From: Computational design of dynamic biosensors for emerging synthetic opioids
A Schematic of the sensor isolation pipeline, including chemical structures of nitazene and menitazene. NitazeneLib1 was screened for sensors using Y2H growth selections in the presence of a ligand of interest. B Binding pocket mutations of PYR1 mutant hits from NitazeneLib1. The most sensitive receptor for each nitazene derivative is shown. Mutations from the wild-type residues are shown in bold. PYR1nita portability to different sensing modalities: Y2H growth assays (C), yeast surface display (Relative Mean PE = mean phycoerythrin (PE) fluorescence normalized to the maximum mean PE value) (D), and in vitro split nano-luciferase assays (E). D, E n = 4, data points represent the mean, error bars represent 1 s.d. and in some cases may be smaller than the symbol. Source Data are provided with this paper within Source Data. Partially created in BioRender. Wheeldon, I. (2025) https://BioRender.com/f7m2ouxhttps://BioRender.com/fq0ih6o.
