Fig. 2: DAB activates TRPM4 independence of Ca²⁺ and temperature.

Representative traces (A), I-V curves (B) and concentration-response relationship curves (C) of DAB-stimulated currents in HEK-293-TRPM4 cells. Currents were recorded in the pipette solution with 0 µM Ca²⁺ (with 5 mM EGTA) at 25 °C. Representative traces (D), I-V curves (E) and concentration-response relationship curves (F) of DAB-stimulated currents recorded in the pipette solution with 1 µM Ca²⁺ at 25 °C. Representative traces (G), I-V curves (H) and concentration-response relationship curves (I) of DAB-stimulated currents recorded in the pipette solution with 0 µM Ca²⁺ (with 5 mM EGTA) at 37 °C. Representative traces (J), I-V curves (K) and concentration-response relationship curves (L) of DAB-stimulated currents recorded in the pipette solution with 1 µM Ca²⁺ at 37 °C. Each data point represents Mean ± SEM in C (n = 8 cells), F (n = 10 cells), I (n = 7 cells) and J (n = 6 cells).