Fig. 2: Perk inactivation partially rescues myelin loss of Jimpy mice. | Nature Communications

Fig. 2: Perk inactivation partially rescues myelin loss of Jimpy mice.

From: Integrated stress response inhibition prolongs the lifespan of a Pelizaeus-Merzbacher disease mouse model by increasing oligodendrocyte survival

Fig. 2

a Representative EM images of axons in corpus callosum from WT mice, Jimpy;Perkfl/fl mice and Jimpy;Perkfl/fl;Olig2-Cre mice at PND 19. b Quantification of the number of myelinated axons in corpus callosum (n = 3 mice/group). p < 0.0001 (WT vs Jimpy;Perkfl/fl, WT vs Jimpy;Perkfl/fl;Olig2-Cre), p = 0.0204 (Jimpy;Perkfl/fl vs Jimpy;Perkfl/fl;Olig2-Cre). c Representative DegenoTag staining of corpus callosum from WT mice, Jimpy;Perkfl/fl mice and Jimpy;Perkfl/fl;Olig2-Cre mice at PND 19. d Quantification of the mean fluorescence intensity (MFI) of DegenoTag marked degenerated axons in corpus callosum (n = 4 WT mice, n = 3 Jimpy;Perkfl/fl mice, n = 3 Jimpy;Perkfl/fl;Olig2-Cre mice). p = 0.0013 (WT vs Jimpy;Perkfl/fl), p = 0.0213 (Jimpy;Perkfl/fl vs Jimpy;Perkfl/fl;Olig2-Cre). e Representative immunoblot analysis for MAG and MBP in the brains from WT mice, Jimpy;Perkfl/fl mice and Jimpy;Perkfl/fl;Olig2-Cre mice at PND 19. f, g Quantitative densitometric analysis for MAG and MBP relative to b-actin (n = 4 mice/group). p < 0.0001 (MAG/actin: WT vs Jimpy;Perkfl/fl, WT vs Jimpy;Perkfl/fl;Olig2-Cre, Jimpy;Perkfl/fl vs Jimpy;Perkfl/fl;Olig2-Cre), p = 0.001 (MBP/actin: WT vs Jimpy;Perkfl/fl), p = 0.001(MBP/actin: WT vs Jimpy;Perkfl/fl;Olig2-Cre), p = 0.008 (MBP/actin: Jimpy;Perkfl/fl vs Jimpy;Perkfl/fl;Olig2-Cre). h Relative mRNA levels of Mbp and Mag from brain tissues of WT mice, Jimpy;Perkfl/fl mice and Jimpy;Perkfl/fl;Olig2-Cre mice at PND 19 (n = 4 mice/group). p = 0.0003 (Mbp: WT vs Jimpy;Perkfl/fl), p = 0.0489 (Mbp: Jimpy;Perkfl/fl vs Jimpy;Perkfl/fl;Olig2-Cre), p = 0.0168 (Mag: WT vs Jimpy;Perkfl/fl), p < 0.0001 (Mag: Jimpy;Perkfl/fl vs Jimpy;Perkfl/fl;Olig2-Cre). Values are mean ± SEM. ns - non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Groups in b and d were compared using one-way ANOVA with Turkey post hoc test. Groups in h was compared using two-way ANOVA with Sidak’s multiple comparison test. Groups in f and g were compared using two-tailed unpaired Welch’s t-test. Source data are provided as a Source Data file.

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