Fig. 7: 2BAct treatment in Jimpy mice rescues inflammation and ISR-related gene sets at PND19. | Nature Communications

Fig. 7: 2BAct treatment in Jimpy mice rescues inflammation and ISR-related gene sets at PND19.

From: Integrated stress response inhibition prolongs the lifespan of a Pelizaeus-Merzbacher disease mouse model by increasing oligodendrocyte survival

Fig. 7

Volcano plots highlighting significant (FDR < 0.05, Log2FC > 0.5) differentially expressed genes in the brains between Jimpy+control diet and WT+control diet (a) and between Jimpy+2BAct diet and Jimpy+control diet at PND 19 (b). c Expression changes in individual genes associated with myelin sheath formation and oligodendrocyte differentiation. *adj-p < 0.05, **adj-p < 0.01, ***adj-p < 0.001 in Jimpy+2BAct diet vs Jimpy+control diet comparison. Gene set enrichment analysis of MSigDB C2 and C5 gene sets (d), and ISR and Hallmark gene sets (e). Volcano plots highlighting ISR CLIC genes in Jimpy+control diet vs WT+control diet (f) and in Jimpy+2BAct diet vs Jimpy+control diet comparisons at PND 19 (g), significantly changed (FDR < 0.05, Log2FC > 0.5) ISR CLIC genes denoted in dark red. n = 3 mice/WT+ control group, n = 4 mice/Jimpy+control group, n = 3 mice/WT+2BAct group, n = 3 mice/Jimpy+control group. For (a, b, f, g), differential expression was determined using a two-sided, moderated t-test with Benjamini–Hochberg adjustment for multiple comparisons. For (d, e), gene set enrichment was analyzed using a two-sided, competitive gene set test CAMERA with Benjamini–Hochberg adjustment. Source data are provided as a Source Data file.

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