Table 2 Comparison of methods for transferring large DNA into mammalian cells
Classification | Transfer methods | Transfer scale | Donor cells | Recipient cells | Efficiency | References |
|---|---|---|---|---|---|---|
Physical and chemical techniques | Polycation-based lipid transfection | Chromosome-level | NA | K562 cells | 1.1–8.7 × 10−5 c | |
1.7 Mb, 2.3 Mb | HT1080 cells | 1-5×10-5 c | ||||
Electroporation | 313 kb | NA | Mouse ES cells | NA | ||
NICE-based microinjection | 1.14 Mb | NA | Mouse MII oocytes | NA | ||
Biological techniques | HVJ-E mediated MMCT | Chromosome-level | CHO | HT1080, hiMSC cells | 3 and 8 times improvement b | |
MV-MMCT | HT1080, hiMSC cells | 50 and 100 times improvement b | ||||
Improved MV-MMCT | HT1080, HFL-1 cells | 2 and 3.7 times improvement d | ||||
MMCT (improved protocol) | HT1080, Hela, hiMSC, mouse ES cells | Maximum 6 times improvement b | ||||
Eco-MMCT | NIH3T3, E14Tg2a, NB2a, CMT-93, Rat-1 cells | 1.3-44 times improvement b | ||||
Am-MMCT | NIH3T3, MCF7, Hela, Cos7, Sirc, E14Tg2a, NB2a, CMT-93, Rat-1 cells | 2.8-58 times improvement b | ||||
Yeast spheroplast-mammalian cell fusion | 1.2 Mb | S. pombe | C127 mouse cells | ~10−6 c | ||
640 kb | S. cerevisiae | RCC-1 cells | ~10−5−10−6 c | |||
210 kb | Goat fetal fibroblasts | |||||
680 kb | LA-9 mouse cells | |||||
600 kb | Mouse L cells | |||||
1.6 Mb | Mouse ES cells | |||||
12 kb-1.1 Mb | HEK293 cells | 326-364 per 3×105 a | ||||
100 kb | HeLa、CHO、A549、Vero cells | 1.5-102 per 3×105 a | ||||
Conjugative plasmid-mediated transfer | 15 kb | E. coli | CHO-K1 cells | 2.6×10−4 c | ||
Ti-plasmid-mediated transfer | 15 kb | A. tumefaciens | HeLa, HEK293, PC12 cells | 17 ± 4、18 ± 6、12 ± 3×10-6 c | ||
Bacterial invasion-mediated transfer | 200 kb | E. coli | HeLa cells | 2.8% a | ||
EBV amplicon vector | 152 kb | NA | Loukes cells | NA | ||
HSV-1amplicon vector | 100 kb | HUES-2, HT1080 cells | 16%、25% a |
