Fig. 9: Folded surface topography suppresses biofilm formation under high shear.
From: Reduction of bacterial colonization on buckling-induced wrinkled surfaces under fluid shear

Optical microscopy images showing biofilm formation of a P. aeruginosa PA14 WT and b S. aureus Newman on flat (F, first and third column) and 5 μm wf⊥ surfaces (second and fourth column) under a shear rate of 200 s−1 at 12 h (first line) and 24 h (second line). Biofilm development was tracked by adding propidium iodide (PI, red signal) to the bacterial suspension, which binds extracellular DNA produced by bacteria during micro-colony formation. Scale bar, 10 μm.