Fig. 5: ISG15 inhibits FASN K48-linked ubiquitination. | Nature Communications

Fig. 5: ISG15 inhibits FASN K48-linked ubiquitination.

From: METTL3 abrogation promotes glioma progression through regulating the ISG15-FASN axis-mediated lipid metabolism in macrophages

Fig. 5: ISG15 inhibits FASN K48-linked ubiquitination.

a FLAG-FASN mutants along with HA-ubiquitin were co-expressed in THP1 cells. Cell lysates and immunoprecipitates were detected with indicated antibodies. (n = 3 independent experiments). b FLAG-FASN mutants co-expressed with HA-ubiquitin in ISG15 silenced THP1 cells treated with MG132. Cell lysates and immunoprecipitates were examined with indicated antibodies. (n = 3 independent experiments). c FLAG-FASN mutants, HA-ubiquitin and Myc-ISG15 or Myc-ISG15ΔGG were co-expressed in THP1 cells treated with MG132. Cell lysates and immunoprecipitates were detected with indicated antibodies. (n = 3 independent experiments). d FLAG-FASN mutants, HA-ubiquitin and Myc-ISG15 or Myc-ISG15ΔGG were co-expressed in THP1 cells treated with MG132. Cell lysates and immunoprecipitates were examined with indicated antibodies. (n = 3 independent experiments). The source data are provided as a Source data file.

Back to article page