Fig. 4: Ca2+ activity enhancers EDN3 and SAK3 promote migration in a collagen gel migration assay. | Nature Communications

Fig. 4: Ca2+ activity enhancers EDN3 and SAK3 promote migration in a collagen gel migration assay.

From: Endothelin-3 and T-type Ca2+ channels drive enteric neural crest cell calcium activity, contractility and migration

Fig. 4: Ca2+ activity enhancers EDN3 and SAK3 promote migration in a collagen gel migration assay.

a E11.5 midgut explants embedded in a collagen matrix and cultured for 3 days in control, EDN3 1 nM, GDNF 10 ng/mL, GDNF + EDN3 1 nM and GDNF + SAK3 10 µM conditions. ENCC migration in the collagen is visible in brightfield Schlieren-type lighting as a halo of radially oriented cells surrounding the darker explant. b Average migration distance from the explant after 3 days in the different drug conditions, two-tailed Mann-Whitney test. Samples numbers in each group, from left to right, are n = 6, 10, 15, 9, 6, 6, 11. Violin plots present the median, upper and lower interquartile range.

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