Fig. 4: The effects on cell viability and colony formation for selected genes, TXN, IRS2, and RHPN2 in three CRC cell lines (HCT116, RKO, SW480). | Nature Communications

Fig. 4: The effects on cell viability and colony formation for selected genes, TXN, IRS2, and RHPN2 in three CRC cell lines (HCT116, RKO, SW480).

From: Mixed-model and transcriptome-wide association analyses identify transcription factors and genes associated with colorectal cancer susceptibility

Fig. 4: The effects on cell viability and colony formation for selected genes, TXN, IRS2, and RHPN2 in three CRC cell lines (HCT116, RKO, SW480).The alternative text for this image may have been generated using AI.

CRC cell lines with knocking down by two sgRNAs for each gene were compared to CRC cell lines with a non-targeting sgRNA control. Cell viability was detected with CK8 assay for TXN (A), IRS2 (D), and RRHPN2 (G). Colony formation assays were performed with cell stained by crystal violet and were further quantified for TXN (B and C), IRS2 (E and F), and RRHPN2 (H and I). The independent experiments were performed in triplicate. P-values were determined by two-sided t test from the comparison of knockdown and control cells. “*”, P < 0.05; “**”, P < 0.01; “***”, P < 0.001. The height of each bar represents the mean value across replicates, and the error bar indicates the standard error of the mean.

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