Fig. 5: On-chip analysis of infected MDA-MB-231 cell–spiked whole blood. | Nature Communications

Fig. 5: On-chip analysis of infected MDA-MB-231 cell–spiked whole blood.

From: A Mechanophenotyping chip for high-throughput detection of metastatic bacteria-infected circulating tumor cells

Fig. 5: On-chip analysis of infected MDA-MB-231 cell–spiked whole blood.The alternative text for this image may have been generated using AI.

a Schematic showing experimental procedure. Whole blood was spiked with MDA-MB-231 cells, stained with Hoechst and invaded by DiO-labeled S. xylosus or L. animalis, and added to the LesM chip. Icons were created in BioRender. Luo, W. (https://BioRender.com/l1e6nxj). b Representative micrographs showing captured MDA-MB-231 cells (Hoechst-stained), intracellular bacteria (DiO-labeled), and blood cells (unstained). Scale bars, 100 μm. c–e Scatter plots of blood cells and spiked S. xylosus –invaded MDA-MB-231 cells, with n = 2194 (biologically independent). Each dot represents one cell. Box plots: median, 25% to 75% boxes, whiskers (-1.5 box to 1.5 box), and outliers indicated. f, g ROC curves of 5 replicated tests for the discrimination of spiked S. xylosus–infected MDA-MB-231 cells by (f) cell area and (g) DiO fluorescence intensity. h–j Scatter plots of blood cells and spiked L. animalis–invaded MDA-MB-231 cells, with n = 2340 (biologically independent). Each dot represents one cell. Box plots: median, 25% to 75% boxes, whiskers (-1.5 box to 1.5 box), and outliers indicated. k, l ROC curves of 5 replicated tests for the discrimination of spiked L. animalis–infected MDA-MB-231 cells by (k) cell area and (l) DiO fluorescence intensity. AUC area under the ROC curve, bac bacteria, BF bright-field microscopy, DiO 3,3’-dioctadecyloxacarbocyanine perchlorate, ROC receiver operating characteristic.

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