Fig. 2: A short region in N-terminus promotes SigmaR1 targeting to ER sheet. | Nature Communications

Fig. 2: A short region in N-terminus promotes SigmaR1 targeting to ER sheet.

From: SigmaR1 is an auxiliary translocon factor with lipid-binding activity that regulates protein and lipid droplet homeostasis

Fig. 2: A short region in N-terminus promotes SigmaR1 targeting to ER sheet.

a–d Representative images of live COS7 cells expressing full-length (a, green) or SigmaR1 truncated mutants including SigmaR1-1-30 (b, green), SigmaR1-10-30 (c, green), SigmaR1∆2-8 (d, green) along with Reticulon4-Halo (top panel; magenta) or Halo-Sec61β (bottom panel; magenta) with insets. e, f Pearson’s correlation coefficient of SigmaR1-GFP (32 cells), SigmaR1∆2-8 (26 cells), SigmaR1-1-30 (30 cells) and SigmaR1-10-30 (32 cells) vs Reticulon4-Halo (e) or Halo-Sec61β [SigmaR1-GFP (28 cells), SigmaR1∆2-8 (20 cells), SigmaR1-1-30 (26 cells) and SigmaR1-10-30 (26 cells)] (f) in more than three independent experiments. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Mean ± SD. g, h Representative images of live COS7 cells expressing SigmaR1(1-30)-Tex2(∆1-517)-GFP (g, green) or SigmaR1(2-8)-Tex2(∆1-473)-GFP (h, green) along with Halo-Tex2 (magenta) and mCh-Sec61β (blue) with insets. i Pearson’s correlation coefficient of two chimeric proteins and Halo-Sec61β [SigmaR1(1-30)-Tex2(∆1-517)-GFP (29 cells) or SigmaR1(2-8)-Tex2(∆1-473)-GFP (24 cells)] as in (g, h) from 3 independent experiments. Two-tailed unpaired Student’s t-test. Mean ± SD. Scale bars, 10 μm in the whole cell images and 2 μm in the insets in (a–d, g, h).

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