Fig. 2: Impaired proliferation of APs in Pqbp1Y65C/Y mice.
a Representative images of WT and Pqbp1Y65C/Y mice cortex stained for Pax6 at E12.5, E15.5, and E17.5. Scale bar, 50 μm. b Quantification of Pax6+ cell numbers in 200 μm cortex for (a). E12.5: n = 5 mice in WT group, n = 6 mice in Y65C group, p = 0.1443; E15.5: n = 4 mice in WT group, n = 4 mice in Y65C group, p = 0.7153; E17.5: n = 4 mice in WT group, n = 5 mice in Y65C group, p = 0.2775. c APs proliferation in WT and Pqbp1Y65C/Y mice cortex was analyzed by BrdU staining. At 1 h after BrdU injection, the proliferation of cells in the E12.5 embryonic stage was analyzed, and the cells that were undergoing cell proliferation were identified. The left panel shows the overall cortex, scale bar 200 μm, while the right panel is an enlarged image of the area within the white square, scale bar 50 μm. d Quantification of the proportion of the APs undergoing cell proliferation for (c). n = 6 mice in WT group, n = 3 mice in Y65C group, p = 0.024. e Representative images of the cortex in WT and Pqbp1Y65C/Y mice at E12.5, stained to calculate the cell cycle. Scale bar, 50 μm. f, g Time course of dual labeling with EdU and BrdU and quantification of APs cell cycle for (e). Ki67 is detected in all proliferating cells. Ts is the length of the S phase of the cell cycle, Tc is the full length of the cell cycle. Ts= Edu+ /Brdu+Edu- xTi, Tc = Ki67+ /Edu+ xTs, Ti = 1.5 h. n = 5 mice in each group, p = 0.0479. h Representative images of WT and Pqbp1Y65C/Y mice cortex stained for pH3, Ki67, and Tbr2 at E12.5. Tbr2 is used to distinguish between VZ and SVZ. Scale bar, 50 μm. i Quantification of the percentage of APs in the M phase for (h). n = 5 mice in each group, p = 0.0001. All quantification data are represented as mean ± SD. b, d, g, i two-tailed unpaired Student’s t test. ns > 0.05, *p ≤ 0.05; ***p ≤ 0.001. Source data are provided as a Source Data file.
