Fig. 1: Experimental flow charts for this study.
From: Flavonoid-mediated bacterial spermidine biosynthesis enhances vitamin accumulation in tomato fruits
A Assay 1: Determination of differential flavonoid components in root exudates of tomato. Assay 2: Exploration of how exogenous flavonoids affect rhizosphere microbiome structure and fruit vitamin accumulation of tomato in natural soil (rutin: naringenin chalcone: kaempferol-3-O-rutinoside = 1:1:1, m/m/m, A: 10 µmol g−1; B: 3 µmol g−1; C: 1 µmol g−1; D: 0.1 µmol g−1; R: rutin, 1 µmol g−1; N: naringenin chalcone, 1 µmol g−1; K: kaempferol-3-O-rutinoside, 1 µmol g−1; Con & SIMYB12: wild-type and SIMYB12-overexpressing plants as controls). Assay 3: Evaluation of the effects of exogenous flavonoids on microbiomes in unplanted natural soil. Assay 4: Analysis of fruit vitamin accumulation in wild-type plants grown in sterilised soil without (Con) and with (C) exogenous flavonoids and in SlMYB12-overexpressing plants. B Isolation key bacteria enriched by flavonoids and evaluation of their role in fruit vitamin accumulation. C Integrated transcriptomic and widely targeted metabolomic analyses, as well as growth, motility and colonisation assays of key bacterial strain under flavonoid treatment. Created in BioRender. fu, w. (https://BioRender.com/y3ukref).
