Fig. 3: PRC2.1 dimer disruption by EPOP in vitro.
From: EPOP restricts PRC2.1 targeting to chromatin by directly modulating enzyme complex dimerization
a Schematic of the co-IP-based dimer disruption assay. FLAG-EZH2 and Myc-EZH2 were co-expressed together with other indicated PRC2 subunits. b Dimer disruption co-IP assay. Subunits of PRC2.1MTF2 were transiently expressed with or without EPOP in HEK293T cells. Representative of two independent replicates. c Mass photometry measurement. Full-length EPOPWT or EPOPD5 was added to the purified PRC2.1MTF2. The mass of major species in solution is indicated. d SEC elution profiles. Full-length EPOP, EPOP(C), and Elongin BC were added to the purified PRC2MTF2 as indicated. Protein standards are shown above the elution profiles.
