Fig. 4: EPOP-mediated inhibition of chromatin binding by PRC2.1 via dimer disruption.
From: EPOP restricts PRC2.1 targeting to chromatin by directly modulating enzyme complex dimerization
a Nuclear extract pull-down assay. Biotinylated CGILhx6 DNA was used as the bait. The bound PRC2.1 was released by enzyme cleavage of a restriction site inserted between biotin and the CGILhx6 sequence. Representative of two independent replicates. b DNA binding EMSA. The serial dilution of PRC2.1MTF2 and PRC2.1MTF2–EPOP is labeled. The dotted red line in the lower panel indicates the position of the DNA– PRC2.1MTF2 supercomplex to compare with that of the DNA–PRC2.1MTF2–EPOP supercomplex. Representative of three independent replicates. c Apparent Kd for DNA binding by the holocomplexes. Graphs display mean ± SEM and are based on three independent replicates. d Nucleosome binding EMSA. The same as (b) except that a mononucleosome was used as the probe. Representative of three independent replicates. e Apparent Kd for nucleosome binding by the holocomplexes. Graphs display mean ± SEM and are based on three independent replicates.
