Fig. 5: Evolutionary dynamics of the sex-determining mechanism in Bb.

TesDwa originated as a duplicate of an ancient autosomal tesD copy, evolving independently after its translocation to the twai locus. TesDwb emerged more recently as a duplicate of an autosomal tesD copy closely resembling the extant tesD (Fig. S10) following a second transposition event. The Bb-b-SDR, containing tesDwb, was subsequently transposed to other autosomal loci, which are no longer transcribed.