Fig. 3: MPXV-specific IgG responses by occupational status and birth cohort.
From: Sero-genomic evidence for occult mpox exposure in healthy Nigerian adults
Serum IgG binding responses to six MPXV antigens measured by Luminex. Antigens represent distinct viral components: A29L, H3L, and M1R (intracellular mature virion, IMV); A35R and B6R (extracellular enveloped virion, EEV); and D6L (soluble antigen). a, b Boxplots of raw and normalized mean fluorescence intensity (MFI) values stratified by a occupational status (healthcare workers [HCW], orange; general population [GenPop], blue) A29L (2208 vs 2550; p = 0.31); A35R (726 vs 503; p = 0.53); B6R (396 vs 255; p = 0.48); D6L (1129 vs 1318; p = 0.56); H3L (1904 vs 1522; p = 0.21) and M1R (401 vs 313 p = 0.0002) and b birth cohort (born before 1980, orange; born in or after 1980, blue); A29L (2948 vs 2161; p = 0.001); A35R (1572 vs 358; p < 0.0001); B6R (708 vs 202; p < 0.0001); D6L (1407 vs 1155; p < =0.24); H3L (2406 vs 1394; p = 0.0008) and M1R (452 vs 305; p < 0.0001). For each antigen, point estimates with 95% confidence intervals displayed above the boxplots indicate the proportion of participants seropositive. Boxes represent the 25th–75th percentiles (interquartile range), the centre line indicates the median, whiskers extend to the most extreme values within 1.5× the interquartile range, and individual points beyond this range are shown as outliers and the black x symbols denote the geometric mean titres (GMTs) for each group. c, d Cumulative magnitude-breadth plots comparing overall antibody responses across groups. Magnitude-breadth scores represent the sum of binding antibody intensities across all six antigens, capturing both the strength (magnitude) and diversity (breadth) of the humoral response. c compares distributions by occupational status (HCW vs GenPop; p = 0.55); d by birth cohort (born before vs after 1980; p < 0.0001). Statistical comparisons were made using the two-tailed Wilcoxon matched-pairs signed-rank test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. IgG, immunoglobulin G; MPXV, mpox virus; IMV, intracellular mature virion; EEV, extracellular enveloped virion; HCWs, healthcare workers; T0, baseline timepoint; T1, follow-up timepoint. Shaded bands represent pointwise exact binomial 95% confidence intervals around the cumulative proportion of participants exceeding each magnitude-breadth score, with the central curve representing the observed cumulative proportion.
