Fig. 4: Increased sleep duration and measures of sleep propensity with loss of beat-Ia in flies and cadm2b in fish.

A Daytime and nighttime arousal threshold (% of flies woken from sleep with a uniform stimulus). One-way ANOVA with Tukey’s multiple comparisons test comparing all genotypes. n, from left to right = 63, 63, 64. B Sleep latency following awakening at lights-on. One-way ANOVA with Tukey’s multiple comparisons test comparing all genotypes. n, from left to right = 21, 42, 34. C Proportion of day, total, and night sleep spent in long ( > 60 min) vs. short ( < 60 min) sleep bouts. Fisher’s exact test, two-sided. D, E Analysis of rest:activity rhythms in beat-Ia RNAi vs controls: D Number of animals categorized as arrhythmic to rhythmic under constant dark conditions. n, from left to right = 59, 60. Chi-square test, ns. E Length of circadian periods in animals under constant conditions. Arrhythmic animals were excluded from analysis. n, from left to right = 42, 45. Unpaired t test, ns. Sleep experiments were run with single-beam DAM monitors (B, D–E) or multibeam DAM monitors (A, C). F–I Sleep measures for control and cadm2b crispant zebrafish. F Mann-Whitney test, two-tailed. G Unpaired t test, two-tailed. n, from left to right = 65, 71. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Each datapoint represents one animal. Bar plots display mean +/- SEM. Source data are provided as a Source Data file.