Fig. 5: beat-Ia acts in development to regulate adult sleep.

Day and night sleep duration (A) and sleep traces (B) from flies expressing beat-Ia RNAi under motor neuron drivers. Two-way ANOVA with Dunnett’s multiple comparisons tests comparing experimental genotypes to control. n, from left to right = 32, 31, 31, 32, 31, 28, 32, 31, 30. C Schematic of temporal restriction of beat-Ia knockdown using a temperature sensitive ubiquitously expressed GAL4 repressor (tubGAL80ts). Day and night sleep duration (D) and sleep traces (E) from flies in which neuronal beat-Ia is knocked down in development or adulthood. Sleep experiments were run with single-beam DAM monitors. Two-way ANOVA with Šídák’s multiple comparisons tests comparing experimental genotypes to control. n, from left to right = 52, 33, 10, 57, 38, 32. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001. Each datapoint represents one animal. Bar plots display mean +/ SEM. Source data are provided as a Source Data file.