Fig. 7: beat-Ia knockdown in NPF cells disrupts synaptic development in the suboesophageal zone (SEZ). | Nature Communications

Fig. 7: beat-Ia knockdown in NPF cells disrupts synaptic development in the suboesophageal zone (SEZ).

From: Cross-species evidence for a developmental origin of adult hypersomnia with loss of synaptic adhesion molecules beat-Ia/CADM2

Fig. 7: beat-Ia knockdown in NPF cells disrupts synaptic development in the suboesophageal zone (SEZ).

Representative images from adult brains of flies expressing membrane reporter CD8::GFP (A) or presynaptic reporter Syt-EGFP (B) under control of NPF-GAL4 with or without beat-Ia RNAi. All images are maximum projections except for SEZ insets of CD8::GFP brains, which are projections of z-stacks cropped to only show SEZ signal. In SEZ insets of Syt-EGFP images (B), beat-Ia RNAi animals show a marked reduction in Syt-EGFP signal. C, D Quantification of Syt-EGFP-tagged puncta across entire brain and in SEZ. Welch’s t test, two-tailed. n, RNAi control = 31, beat-Ia RNAi = 38. E Quantification of the presence or absence of clusters of Syt-EGFP-tagged synapses in the SEZ, illustrated in (B). Fisher’s exact test, two-sided. Scale bar = 50 µM. *** p ≤ 0.001, **** p ≤ 0.0001. Each datapoint represents one animal. Bar plots display mean +/- SEM. Source data are provided as a Source Data file.

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