Fig. 2: The effect of G:U pairs within the seed on miRNA binding affinity. | Nature Communications

Fig. 2: The effect of G:U pairs within the seed on miRNA binding affinity.

From: Biochemical principles of miRNA targeting in flies

Fig. 2: The effect of G:U pairs within the seed on miRNA binding affinity.

a KD values for canonical seed-matched sites (left: t2–t8 targets with t1A; right: t2–t8 targets) and one, two and three G:U pairs within let-7 seed at indicated positions. Shown for comparison: 8mer (t2–t8 site with t1A; in cyan) and 7mer-m8 (t2–t8 site; in orange). Error bars indicate 95% CI on the median from 2000 independent MLE runs. B represents C, G, or U. The equilibrium dissociation constant of let-7-loaded Ago1 for the competitor bearing G:U base-pairs (b) or mismatches (c) within the seed, relative to that of a fully complementary target. miRNA sequence shown in red. Data are mean ± SD for ≥three independent experiments. Source data are provided as a Source data file.

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