Fig. 2: Deletion and insertion mutants both exhibit a loss of early brk expression as well as a reduction in the broad expression pattern observed at later stages.
From: Inferring chromatin architecture at a single locus through probabilistic in situ DNA localization
A Schematic of the brk locus highlighting the 800 bp deletion of the proximal PPE (Δ800PPE), deletions of E1 (ΔE1) or E2 (ΔE2) enhancers, and the increase in distance between the E1 and PPE by 7.3 kB through the MiMIC insertion. B-F” HCR against brk transcript in WT (B-B”), Δ800PPE mutant (C-C”), MiMIC insertion (D-D”), ΔE1 (E-E”), and ΔE2 mutant (F-F”) embryos at nc13 (top), nc14 early (middle), and nc14 late (bottom). Scale bar corresponds to 50μm. G Average intensity measurements of brk transcripts detected by HCR for indicated genotypes at nc13 (WT n = 12, Δ800PPE mutant n = 10, MiMIC insertion n = 12, ΔE1 n = 10, ΔE2 n = 10), nc14 early (WT n = 14, Δ800PPE n = 12, MiMIC n = 10 and ΔE1 n = 10, ΔE2 n = 11) and nc14 late (WT n = 12, Δ800PPE n = 11, MiMIC n = 10, ΔE1 n = 11 and ΔE2 n = 9). H Average width measurements of brk pattern at nc14 early and nc14 late. Statistical significance was assessed using one-way ANOVA followed by Tukey’s Honest Significant Difference (HSD) test for multiple comparisons. Mean ± SD is shown in black. See also Fig. S2 and Source Data File for p-values and significant differences.
