Fig. 1: WPG stimulates the myeloid compartment to enhanced vaccine responses.
Single-cell RNA sequencing data, previously published40, were re-analysed from mice given PBS (n = 3) or WPG (n = 3) (sacrificed at day 3 and day 7 post WPG treatment). A Two-dimensional UMAP representation of 4803 cells from the three treatment groups aggregated together. B UMAP dimension reduced of PBS (1251 cells), WPG 3(2161) and WPG7 (1385). C Pathway impact analysis on DEG of clusters 7, 11 and 12. Pathway significance was assessed using a two-sided hypergeometric test with FDR correction D Comparison between WPG and PBS in the average expression of antigen presentation genes from clusters 11, 7 and 12. Significance was assessed using a two-sided hypergeometric test; topology based on degree centrality E Single cell RNA sequencing data showing a UMAP of expression of Ctss, H2-Aa, H2-Ab1 and B2m in all clusters in WPG. F Schematic of treatment dosage of mice vaccinated with PC (against SIINFEKL) (Created in BioRender. Hamdan, F. (https://BioRender.com/otyn63b). G Flow-cytometry data showing a UMAP dimension of different CD8+ memory cells from splenocytes of PBS and WPG treated mice after stimulating with SIINFEKL peptide at day 15. Expression of IL-2, TNF-α and IFN-γ were also mapped in the UMAP obtained from n = 3 mice from each treatment group. H IFN-γ levels of splenocytes of PBS and WPG treated mice after stimulation with media, Ad5/3 and SIINFEKL peptide obtained from n = 3 mice from each treatment group (‘****’ p < 0.0001; ‘**’ p = 0.0023). Data are the means ± SD. Contour plots are representative images of splenocytes stimulated with SIINFEKL peptide. I TNF-α levels of splenocytes of PBS and WPG treated mice after stimulation with PBS, Ad5/3 and SIINFEKL peptide obtained from n = 3 mice from each treatment group (‘****’ p < 0.0001; ‘***’ p = 0.0002). Data are the means ± SD. Contour plots are representative images of splenocytes stimulated with SIINFEKL peptide. Statistical significance was assessed with one-way ANOVA for the flow cytometry analysis along with a Tukey’s multiple comparisons test. Source data are provided as a Source Data file.
