Fig. 2: Synthesis and characterization of IL/aC@RBC and BaO2.
From: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy
a Schematic showing the synthesis process of IL/aC@RBC. b SDS-PAGE protein analysis of nRBC, RBC, IL/aC@RBC, IL-12, and aCTLA-4. Marker indicates the molecular weight in kilodaltons (kDa). c Representative CLSM images of IL/aC@RBC. RBCM, IL-12, and aCTLA-4 were respectively labeled with DiI (red), AMCA (blue), and FITC (green). Scale bar, 2 μm. d Fluorescence colocalization spectra of RBCM-DiI, IL-12-AMCA, and aCTLA-4-FITC in IL/aC@RBC, as shown in (c). e SEM images of nRBC and IL/aC@RBC. Scale bar, 2 μm. f Relative enzyme activity analysis of CAT in nRBC, RBC, IL/aC@RBC and RBCM. g In vitro O2 production from nRBC and IL/aC@RBC. h IFN-γ concentrations secreted by murine splenocytes subjected to IL-12 and IL@RBC. i High-resolution TEM images of BaO2. Scale bars, 100 nm and 20 nm. j XPS spectrum of BaO2. k Release profiles of Ba2+ from BaO2 at different pH values. l H2O2 generation from BaO2 at different pH values. Experiments in (b, c, e, i) were independently repeated three times with comparable results. Data in (f, g, h, k, l) were presented as mean ± SD. n = 3 independent experiments. Statistical significance was determined using two-way ANOVA (k) and two-sided unpaired student’s t-test (l). Source data are provided as a Source Data file.
