Fig. 9: RT + IL/aC@RBAH potentiates systemic antitumor immunity in subcutaneous Panc02 tumors.

a Representative immunofluorescence images of CD8⁺ T cells and CD4⁺ T cells in tumors from Panc02 tumor-bearing mice after different treatments. Scale bar, 200 µm. b, c Quantifications of CD8⁺ T cells (b) and CD4⁺ T cells (c) in tumors from subcutaneous Panc02 tumor-bearing mice corresponding to immunofluorescence images in (a). d–i Representative flow cytometry plots and quantifications of IFN-γ⁺ cells gating on CD3⁺CD4⁺ T cells (d, e), CD3⁺CD8⁺ T cells (f, g), and NK cells (h, i) in spleens from Panc02 tumor-bearing mice after different treatments. G1, Saline; G2, RT; G3, RT + RBAH; G4, RT + aC@RBAH; G5, RT + IL@RBAH; G6, RT + IL/aC@RBAH. Experiments in (a, d, f, h) were independently repeated three times with comparable results. Data in (b, c, e, g, i) were presented as mean ± SD. n = 3 mice per group. Statistical significance was determined using two-sided unpaired student’s t-test (b, c, e, g, i). Source data are provided as a Source Data file.