Fig. 1: Diversity of energy resource and metabolism of immune cells in the blood.
From: Spatiotemporal regulation of energetic charge dictates T cell function
Pipeline for SPICE-Met analyses: a method that uses transgenic mice expressing a fluorescent reporter for ATP:ADP ratio to profile energy metabolism in complex cell populations. A Experimental scheme. Immune cell populations were isolated from blood of Vav-Cre/Percevalfl/fl (SPICY) mice, and metabolic parameters were addressed by SPICE-Met flow cytometry. B Oligomycin (Oligo) treatment was used to reveal OXPHOS/glycolysis relative and net contributions to the generation of cellular energy resources. C Iodoacetic acid (IA) treatment was used to reveal glucose/fatty, amino acids, relative and net contributions into the generation of cellular energy resources. D–F Diversity of ATP:ADP ratio and energy metabolism in various immune cell populations from the blood. D ATP:ADP ratio measured for the indicated populations. E Heatmaps representing the net and relative contribution (dependency) of OXPHOS, glycolysis, glucose, and FA/AA for the indicated cell population. F Quantification of the net contribution of OXPHOS, glycolysis, glucose and FA/AA for the indicated cell population. Compiled from 21 independent experiments with a total of 24 mice. G Correlation analysis of ATP:ADP ratio of blood lymphocytes with different metabolic parameters. Compiled from 21 independent experiments with a total of 24 mice. Each dot represents the value obtained in one mouse.
